Phosphate Buffer System

H2PO4 ⇌ HPO42− + H+

ภาพรวม

The dihydrogen phosphate/hydrogen phosphate buffer system operates near pH 7.2, making it ideal for biological applications. With pKa2 = 7.2, it buffers effectively between pH 6.2 and 8.2. This is the buffer system used in phosphate-buffered saline (PBS), the most common buffer in biology.

ผู้เข้าร่วม

บทบาท สาร สัมประสิทธิ์ สถานะ
สารตั้งต้น Phosphoric Acid H₃PO₄ 1 (aq)

ตัวอย่างในชีวิตประจำวัน

Phosphate buffers help maintain the pH of many biological fluids and are used in nearly every molecular biology laboratory.

ความสำคัญทางอุตสาหกรรม

The phosphate buffer system is used in PBS for cell culture, in clinical diagnostics, in food processing, and in pharmaceutical formulations requiring near-neutral pH.

Frequently Asked Questions

What is the equation for Phosphate Buffer System?
The balanced equation is: H₂PO₄⁻ ⇌ HPO₄²⁻ + H⁺.
What type of reaction is Phosphate Buffer System?
Phosphate Buffer System is a acid-base reaction. It is reversible under certain conditions.
Is Phosphate Buffer System exothermic or endothermic?
Phosphate Buffer System is endothermic (absorbs energy).