Sudaki Demir Tayini

Tiyosiyanat kolorimetrisi ile çözünmüş demir konsantrasyonunu ölçme

Colorimetry Intermediate (High School) 75 dak ~$10,00

Hedef

Su örneğindeki demir(III) iyonu konsantrasyonunu tiyosiyanat kolorimetrik yöntemiyle belirlemek.

Arka Plan

Iron(III) ions react with thiocyanate ions (SCN⁻) to form the intensely red-colored complex [Fe(SCN)]²⁺. The intensity of the red color is proportional to the Fe³⁺ concentration, following the Beer-Lambert law. This method is widely used in water quality testing and environmental monitoring. Students learn both the analytical technique and the chemistry of complex ion formation.

Güvenlik Uyarıları

  • FeCl₃ solution stains skin and clothing
  • KSCN is harmful if swallowed
  • HNO₃ is corrosive — handle with care
  • Dispose of thiocyanate waste in designated containers

Gerekli KKD

goggles gloves lab_coat

Malzemeler

  • Iron(III) chloride (FeCl₃) standard (100 mL)
    0.01 M stock solution
  • Potassium thiocyanate (KSCN) (100 mL)
    0.1 M solution
  • Dilute HNO₃ (0.1 M) (50 mL)
    To maintain acidity
  • Water sample (50 mL)
    Unknown iron content
  • Distilled water (500 mL)
    For dilutions

Ekipman

Colorimeter or spectrophotometer Cuvettes (matched pair) Volumetric flasks (50 mL, 7 total) Pipettes (1 mL, 5 mL, 10 mL) Pipette filler

Prosedür

1

Prepare a series of standard Fe³⁺ solutions (0, 1, 2, 4, 6, 8, 10 ppm) by diluting the stock FeCl₃ solution in 50 mL volumetric flasks.

15 dak
2

Add 5 mL of 0.1 M KSCN and 2 mL of dilute HNO₃ to each standard flask and the water sample. Make up to volume with distilled water.

10 dak
3

Allow 5 minutes for full color development. The solutions should range from pale to deep red.

5 dak
4

Set the colorimeter to 480 nm. Zero with the blank solution (0 ppm Fe³⁺ + KSCN).

5 dak
5

Measure the absorbance of each standard solution and the water sample.

15 dak
6

Plot the calibration curve (absorbance vs. concentration). Use it to determine the iron concentration in the water sample.

15 dak
7

Calculate the iron concentration and evaluate whether the water meets drinking water standards (<0.3 ppm).

10 dak

Beklenen Sonuçlar

The calibration curve should be linear from 0 to 10 ppm. The deep red color of [Fe(SCN)]²⁺ intensifies with increasing Fe³⁺ concentration. The water sample's iron content is determined from the calibration curve.

Temizlik

Collect all thiocyanate-containing solutions in labeled waste. Rinse all glassware thoroughly. Wipe down the colorimeter.

Ayrıntılar

Kategori
Colorimetry
Zorluk
Intermediate (High School)
Süre
75 dak
Tahmini Maliyet
$10,00
Adımlar
7
Malzemeler
5

Daha Fazla Deney

Aspirinin Spektrofotometrik Tayini 75 min Beer-Lambert Yasasının Doğrulanması 60 min Fosfat Kolorimetrik Tayini 90 min Glukozun Kolorimetrik Tayini 60 min Karışımın Spektrofotometrik Analizi 75 min Kolorimetrik Protein Analizi (Bradford Yöntemi) 60 min Spektrofotometreyle Gıda Boyası Analizi 60 min

Frequently Asked Questions

What is the objective of Sudaki Demir Tayini?
Su örneğindeki demir(III) iyonu konsantrasyonunu tiyosiyanat kolorimetrik yöntemiyle belirlemek.
How difficult is Sudaki Demir Tayini?
This experiment is rated as Intermediate (High School). It takes approximately 75 minutes to complete.
What safety precautions are needed for Sudaki Demir Tayini?
Key safety precautions include: FeCl₃ solution stains skin and clothing; KSCN is harmful if swallowed; HNO₃ is corrosive — handle with care.
What materials are needed for Sudaki Demir Tayini?
The main materials required are: Iron(III) chloride (FeCl₃) standard, Potassium thiocyanate (KSCN), Dilute HNO₃ (0.1 M), Water sample, Distilled water.
What results should I expect from Sudaki Demir Tayini?
The calibration curve should be linear from 0 to 10 ppm. The deep red color of [Fe(SCN)]²⁺ intensifies with increasing Fe³⁺ concentration. The water sample's iron content is determined from the calibration curve.