Analisis Pewarna Makanan dengan Spektrofotometri

Mengidentifikasi pewarna makanan buatan dari spektra absorbansinya

Colorimetry Intermediate (High School) 60 menit ~$8,00

Tujuan

Tentukan identitas dan konsentrasi pewarna makanan buatan dalam minuman komersial dengan mengukur spektra absorbansinya.

Latar Belakang

Artificial food dyes have characteristic absorption spectra that serve as fingerprints for identification. FD&C Red No. 40 absorbs maximally at 504 nm, Blue No. 1 at 630 nm, and Yellow No. 5 at 428 nm. By scanning the absorption spectrum of a commercial beverage and comparing peak wavelengths with known dye spectra, students can identify which dyes are present and estimate their concentrations.

Peringatan Keselamatan

  • Food dyes can stain clothing
  • Do not drink any samples after use in the lab
  • Handle cuvettes by the frosted sides only

APD yang Diperlukan

goggles lab_coat

Bahan

  • Standard food dye solutions (50 mL each)
    Red 40, Blue 1, Yellow 5 (0.01%)
  • Commercial beverages (50 mL each)
    2–3 colored drinks
  • Distilled water (200 mL)
    For dilutions and blank

Peralatan

UV-Vis spectrophotometer (or colorimeter with multiple filters) Cuvettes (quartz or plastic) Pipettes Volumetric flasks (25 mL, 4 total)

Prosedur

1

If using a spectrophotometer, scan each standard dye solution from 400 to 700 nm to identify the λmax (wavelength of maximum absorbance).

10 menit
2

Record the λmax for each standard: Red 40 (≈504 nm), Blue 1 (≈630 nm), Yellow 5 (≈428 nm).

5 menit
3

Dilute commercial beverage samples if too concentrated (absorbance should be below 1.0). Record all dilution factors.

10 menit
4

Scan each beverage sample from 400 to 700 nm. Record the absorption spectrum.

15 menit
5

Compare the beverage absorption peaks with the standard dye λmax values to identify which dyes are present.

10 menit
6

If a calibration curve was prepared for a specific dye, calculate the dye concentration in each beverage (accounting for dilution).

10 menit

Hasil yang Diharapkan

Each beverage should show absorption peaks matching one or more standard food dyes. Green drinks typically contain both Blue 1 and Yellow 5. Orange drinks often contain Red 40 and Yellow 5.

Pembersihan

Pour dilute dye solutions down the drain with running water. Rinse cuvettes immediately to prevent staining.

Detail

Kategori
Colorimetry
Tingkat Kesulitan
Intermediate (High School)
Durasi
60 menit
Perkiraan Biaya
$8,00
Langkah
6
Bahan
3

Eksperimen Lainnya

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Frequently Asked Questions

What is the objective of Analisis Pewarna Makanan dengan Spektrofotometri?
Tentukan identitas dan konsentrasi pewarna makanan buatan dalam minuman komersial dengan mengukur spektra absorbansinya.
How difficult is Analisis Pewarna Makanan dengan Spektrofotometri?
This experiment is rated as Intermediate (High School). It takes approximately 60 minutes to complete.
What safety precautions are needed for Analisis Pewarna Makanan dengan Spektrofotometri?
Key safety precautions include: Food dyes can stain clothing; Do not drink any samples after use in the lab; Handle cuvettes by the frosted sides only.
What materials are needed for Analisis Pewarna Makanan dengan Spektrofotometri?
The main materials required are: Standard food dye solutions, Commercial beverages, Distilled water.
What results should I expect from Analisis Pewarna Makanan dengan Spektrofotometri?
Each beverage should show absorption peaks matching one or more standard food dyes. Green drinks typically contain both Blue 1 and Yellow 5. Orange drinks often contain Red 40 and Yellow 5.