Titrasi Vitamin C dengan Iodin

Mengukur kandungan asam askorbat dalam jus buah

Titrations Intermediate (High School) 40 menit ~$10,00

Tujuan

Tentukan kandungan vitamin C (asam askorbat) jus buah segar menggunakan titrasi iodin dengan indikator pati.

Latar Belakang

Ascorbic acid reduces iodine to iodide ions in a 1:1 molar ratio. Starch indicator turns dark blue-black in the presence of excess iodine. When all the vitamin C is consumed, the next drop of iodine produces the blue-black color, marking the endpoint.

Peringatan Keselamatan

  • Iodine stains skin and clothing
  • Wear safety goggles
  • Handle iodine solution with care

APD yang Diperlukan

goggles lab_coat

Bahan

  • Fresh orange juice (200 mL)
    Freshly squeezed preferred
  • Iodine solution (100 mL)
    0.005M
  • Starch indicator solution (10 mL)
    1% solution
  • Distilled water (200 mL)

Peralatan

50 mL burette Burette stand and clamp 250 mL Erlenmeyer flask 25 mL pipette Pipette filler White tile

Prosedur

1

Rinse and fill the burette with the standardized iodine solution. Record the initial volume.

3 menit Iodine stains
2

Pipette 25.0 mL of orange juice into the Erlenmeyer flask.

2 menit
3

Add 5 drops of starch indicator to the flask.

1 menit
4

Titrate by adding iodine solution slowly while swirling. The brown iodine color will disappear as it reacts with vitamin C.

8 menit
5

Near the endpoint, add drop by drop until a persistent dark blue-black color appears for 20 seconds.

3 menit
6

Record the final volume and repeat for concordant results.

15 menit
7

Calculate the mass of vitamin C per volume of juice. Compare with the labeled value.

5 menit

Hasil yang Diharapkan

Fresh orange juice typically contains 40-80 mg of vitamin C per 100 mL. The starch-iodine endpoint should be a sharp transition to blue-black. Older or heat-treated juice will show lower vitamin C content.

Pembersihan

Dispose of iodine waste in designated containers. Rinse all glassware with water. Clean any iodine stains with sodium thiosulfate solution.

Detail

Kategori
Titrations
Tingkat Kesulitan
Intermediate (High School)
Durasi
40 menit
Perkiraan Biaya
$10,00
Langkah
7
Bahan
4

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Frequently Asked Questions

What is the objective of Titrasi Vitamin C dengan Iodin?
Tentukan kandungan vitamin C (asam askorbat) jus buah segar menggunakan titrasi iodin dengan indikator pati.
How difficult is Titrasi Vitamin C dengan Iodin?
This experiment is rated as Intermediate (High School). It takes approximately 40 minutes to complete.
What safety precautions are needed for Titrasi Vitamin C dengan Iodin?
Key safety precautions include: Iodine stains skin and clothing; Wear safety goggles; Handle iodine solution with care.
What materials are needed for Titrasi Vitamin C dengan Iodin?
The main materials required are: Fresh orange juice, Iodine solution, Starch indicator solution, Distilled water.
What results should I expect from Titrasi Vitamin C dengan Iodin?
Fresh orange juice typically contains 40-80 mg of vitamin C per 100 mL. The starch-iodine endpoint should be a sharp transition to blue-black. Older or heat-treated juice will show lower vitamin C content.