Firefly Bioluminescence (Luciferin Oxidation)
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Luciferin + ATP + O2 → Oxyluciferin + AMP + PPi + CO2 + hν
Visão geral
Firefly luciferase catalyzes the ATP-dependent oxidation of luciferin, producing oxyluciferin in an electronically excited state that emits yellow-green light (560 nm). This is the most efficient bioluminescent system known, converting about 88% of chemical energy to light with almost no heat waste.
Participantes
| Papel | Substância | Coeficiente | Estado |
|---|---|---|---|
| Produto | Carbon Dioxide CO₂ | 1 | (g) |
Exemplo do cotidiano
Fireflies flashing on summer evenings use this reaction to communicate with potential mates, each species having a unique flash pattern.
Importância industrial
Os ensaios repórter de luciferase são indispensáveis na investigação biomédica, descoberta de fármacos e diagnóstico clínico. O gene da luciferase é o gene repórter mais amplamente utilizado em biologia molecular.
Propriedades
- Tipo
- Photochemical
- Reversível
- Não
- Energia
- Exotérmico
- Catalisador
- Luciferase enzyme