Phosphate Buffer System
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H2PO4− ⇌ HPO42− + H+
Visão geral
The dihydrogen phosphate/hydrogen phosphate buffer system operates near pH 7.2, making it ideal for biological applications. With pKa2 = 7.2, it buffers effectively between pH 6.2 and 8.2. This is the buffer system used in phosphate-buffered saline (PBS), the most common buffer in biology.
Participantes
| Papel | Substância | Coeficiente | Estado |
|---|---|---|---|
| Reagente | Phosphoric Acid H₃PO₄ | 1 | (aq) |
Exemplo do cotidiano
Phosphate buffers help maintain the pH of many biological fluids and are used in nearly every molecular biology laboratory.
Importância industrial
O sistema tampão fosfato é utilizado em PBS para cultura celular, em diagnósticos clínicos, no processamento alimentar e em formulações farmacêuticas que requerem pH próximo do neutro.
Propriedades
- Tipo
- Acid-Base
- Reversível
- Sim
- Energia
- Endotérmico