Phosphate Buffer System

H2PO4 ⇌ HPO42− + H+

Visão geral

The dihydrogen phosphate/hydrogen phosphate buffer system operates near pH 7.2, making it ideal for biological applications. With pKa2 = 7.2, it buffers effectively between pH 6.2 and 8.2. This is the buffer system used in phosphate-buffered saline (PBS), the most common buffer in biology.

Participantes

Papel Substância Coeficiente Estado
Reagente Phosphoric Acid H₃PO₄ 1 (aq)

Exemplo do cotidiano

Phosphate buffers help maintain the pH of many biological fluids and are used in nearly every molecular biology laboratory.

Importância industrial

O sistema tampão fosfato é utilizado em PBS para cultura celular, em diagnósticos clínicos, no processamento alimentar e em formulações farmacêuticas que requerem pH próximo do neutro.

Frequently Asked Questions

What is the equation for Phosphate Buffer System?
The balanced equation is: H₂PO₄⁻ ⇌ HPO₄²⁻ + H⁺.
What type of reaction is Phosphate Buffer System?
Phosphate Buffer System is a acid-base reaction. It is reversible under certain conditions.
Is Phosphate Buffer System exothermic or endothermic?
Phosphate Buffer System is endothermic (absorbs energy).