Chemistry Experiments

95 experiment protocols with step-by-step instructions

All Categories Flame Tests Titrations Electrochemistry Organic Synthesis Crystallization Gas Laws Thermochemistry Qualitative Analysis Colorimetry Classroom Demonstrations
All Levels Beginner (Middle School) Intermediate (High School) Advanced (University) Professional (Lab)

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Beer-Lambert Law Verification

Demonstrating the linear relationship between absorbance and concentration

Verify the Beer-Lambert law by measuring the absorbance of CuSO₄ solutions at various concentrations and constructing a calibration curve.

Colorimetry · Intermediate (High School) · 60 min · 7 steps

Colorimetric Determination of Glucose

Measuring blood glucose analog using the DNS method

Determine glucose concentration using the dinitrosalicylic acid (DNS) colorimetric method and understand reducing sugar chemistry.

Colorimetry · Intermediate (High School) · 60 min · 7 steps

Determination of Iron in Water

Measuring dissolved iron concentration using thiocyanate colorimetry

Determine the concentration of iron(III) in a water sample using the thiocyanate colorimetric method.

Colorimetry · Intermediate (High School) · 75 min · 7 steps

Food Dye Analysis by Spectrophotometry

Identifying artificial food colors by their absorption spectra

Determine the identity and concentration of artificial food dyes in commercial beverages by measuring their absorption spectra.

Colorimetry · Intermediate (High School) · 60 min · 6 steps

Crystallization of Salol (Phenyl Salicylate)

Observing crystal growth under a microscope in real time

Observe the real-time growth of salol crystals under a microscope, studying nucleation, crystal growth rates, and the effect of temperature …

Crystallization · Intermediate (High School) · 30 min · 6 steps

Recrystallization of Benzoic Acid

Purifying a solid by dissolving in hot solvent and cooling

Purify impure benzoic acid by recrystallization from hot water, demonstrating the standard purification technique for organic solids.

Crystallization · Intermediate (High School) · 45 min · 8 steps

Supersaturation and Instant Crystallization

Triggering instant crystal formation in sodium acetate

Prepare a supersaturated sodium acetate solution and trigger instantaneous crystallization, demonstrating metastable supersaturation and exothermic crystallization (hot ice).

Crystallization · Intermediate (High School) · 35 min · 6 steps

Chemiluminescence with Luminol

Producing blue light through a chemical reaction without heat

Demonstrate chemiluminescence by oxidizing luminol with hydrogen peroxide in the presence of a catalyst, producing visible blue light.

Classroom Demonstrations · Intermediate (High School) · 30 min · 6 steps

Iodine Clock Reaction

A timed reaction that suddenly turns dark blue after a predictable delay

Demonstrate reaction kinetics and the effect of concentration and temperature on reaction rate using the iodine clock reaction.

Classroom Demonstrations · Intermediate (High School) · 45 min · 7 steps

Daniell Cell Construction

Building a classic zinc-copper galvanic cell

Construct a Daniell cell to measure the standard cell potential and observe the electrochemical reactions at each electrode.

Electrochemistry · Intermediate (High School) · 45 min · 8 steps

Electrolysis of Copper Sulfate

Quantitative electrolysis with copper electrodes

Perform quantitative electrolysis of copper sulfate solution using copper electrodes and verify Faraday's first law of electrolysis.

Electrochemistry · Intermediate (High School) · 50 min · 7 steps

Electrolysis of Water

Splitting water into hydrogen and oxygen gases

Decompose water into hydrogen and oxygen gases using electrolysis, and verify the 2:1 volume ratio predicted by stoichiometry.

Electrochemistry · Intermediate (High School) · 40 min · 7 steps

Electroplating with Copper

Depositing a thin layer of copper onto a metal object

Electroplate a metal key or coin with copper, demonstrating the industrial process of electrodeposition and Faraday's laws.

Electrochemistry · Intermediate (High School) · 60 min · 8 steps

Barium Flame Test

Identifying barium by its yellow-green flame

Observe the pale yellow-green flame produced by barium ions and understand why barium compounds require extra safety precautions.

Flame Tests · Intermediate (High School) · 20 min · 6 steps

Flame Test Comparison Lab

Testing multiple metals to build a flame color reference chart

Perform flame tests on six different metal salts to create a reference chart of characteristic flame colors for qualitative analysis.

Flame Tests · Intermediate (High School) · 45 min · 9 steps

Boyle's Law Verification

Demonstrating the inverse relationship between pressure and volume

Verify Boyle's law (P1V1 = P2V2) by measuring the volume of a trapped gas at different pressures.

Gas Laws · Intermediate (High School) · 40 min · 5 steps

Diffusion Rate Comparison (Graham's Law)

Comparing diffusion rates of HCl and NH₃ gases

Verify Graham's law of diffusion by comparing the rates at which HCl and NH₃ gases travel through a glass tube.

Gas Laws · Intermediate (High School) · 30 min · 6 steps

Gas Stoichiometry with Magnesium

Calculating molar volume by reacting Mg with HCl

Determine the molar volume of hydrogen gas at room conditions by reacting a known mass of magnesium ribbon with excess …

Gas Laws · Intermediate (High School) · 40 min · 7 steps

Hydrogen Gas Collection

Generating and collecting hydrogen from zinc and acid

Generate hydrogen gas from the reaction of zinc with hydrochloric acid and collect it by water displacement.

Gas Laws · Intermediate (High School) · 35 min · 7 steps

Molar Volume of a Gas at STP

Experimentally determining 22.4 L/mol using butane

Determine the molar volume of butane gas at STP by measuring the mass and volume of gas released from a …

Gas Laws · Intermediate (High School) · 35 min · 8 steps

Biodiesel Production from Vegetable Oil

Converting cooking oil to biodiesel via transesterification

Convert vegetable oil to biodiesel (fatty acid methyl esters) by base-catalyzed transesterification with methanol.

Organic Synthesis · Intermediate (High School) · 60 min · 8 steps

Making Nylon 6,10 (Nylon Rope Trick)

Pulling a continuous strand of nylon from an interface

Synthesize nylon 6,10 at the interface of two immiscible solutions and pull a continuous strand from the beaker.

Organic Synthesis · Intermediate (High School) · 30 min · 6 steps

Soap Making by Saponification

Converting fats into soap using sodium hydroxide

Make soap by the saponification of vegetable oil with sodium hydroxide, demonstrating the hydrolysis of ester bonds in triglycerides.

Organic Synthesis · Intermediate (High School) · 60 min · 8 steps

Anion Identification Tests

Detecting common anions through selective chemical reactions

Identify unknown anions using specific precipitation, acid-decomposition, and redox tests.

Qualitative Analysis · Intermediate (High School) · 60 min · 7 steps

Borax Bead Test for Metal Ions

Identifying transition metals by colored glass beads formed in a borax fusion

Identify transition metal ions by the characteristic colors they produce when fused into a borax (sodium tetraborate) glass bead.

Qualitative Analysis · Intermediate (High School) · 45 min · 6 steps

Cation Identification by Precipitation

Systematic separation of Group I–V cations using selective reagents

Identify unknown cations in a solution using the classical qualitative analysis scheme with group reagents.

Qualitative Analysis · Intermediate (High School) · 90 min · 7 steps

Chromatography of Plant Pigments

Separating chlorophyll and carotenoids using paper chromatography

Separate and identify plant pigments using paper chromatography, and calculate Rf values for each pigment.

Qualitative Analysis · Intermediate (High School) · 60 min · 6 steps

Unknown Substance Identification Challenge

Using physical and chemical properties to identify mystery powders

Apply multiple qualitative tests to identify unknown white powders, developing systematic analytical thinking skills.

Qualitative Analysis · Intermediate (High School) · 75 min · 8 steps

Bomb Calorimetry Simulation

Measuring the energy content of food using calorimetry

Measure the energy (calorie) content of different food items by burning them and measuring the heat transferred to water.

Thermochemistry · Intermediate (High School) · 45 min · 8 steps

Coffee Cup Calorimetry

Measuring enthalpy of neutralization using a simple calorimeter

Determine the enthalpy of neutralization of HCl and NaOH using a polystyrene cup calorimeter.

Thermochemistry · Intermediate (High School) · 40 min · 6 steps

Endothermic Reaction Demonstration

Barium hydroxide and ammonium thiocyanate cold reaction

Demonstrate a dramatically endothermic reaction where the mixture becomes cold enough to freeze water on the outside of the flask.

Thermochemistry · Intermediate (High School) · 20 min · 7 steps

Exothermic Crystallization of Sodium Acetate

Measuring the heat released during instant crystallization

Quantify the heat released during the crystallization of supersaturated sodium acetate using calorimetry.

Thermochemistry · Intermediate (High School) · 30 min · 6 steps

Specific Heat Capacity of Metals

Comparing heat capacities of aluminum, copper, and iron

Determine the specific heat capacity of several metals using the method of mixtures and compare with accepted values.

Thermochemistry · Intermediate (High School) · 40 min · 7 steps

Acid-Base Titration with Methyl Orange

Titrating a weak base with a strong acid

Determine the concentration of a sodium carbonate solution by titrating it with hydrochloric acid using methyl orange indicator, suitable for …

Titrations · Intermediate (High School) · 40 min · 7 steps

Acid-Base Titration with Phenolphthalein

Determining the concentration of an acid using a standard base

Determine the concentration of an unknown hydrochloric acid solution by titrating it with a standardized sodium hydroxide solution, using phenolphthalein …

Titrations · Intermediate (High School) · 45 min · 8 steps

Back Titration of Antacid Tablets

Measuring the acid-neutralizing capacity of commercial antacids

Determine the acid-neutralizing capacity of a commercial antacid tablet using a back titration method.

Titrations · Intermediate (High School) · 60 min · 8 steps

pH Titration Curve

Plotting a complete acid-base titration curve with pH meter

Record pH measurements during an acid-base titration and construct a complete S-shaped titration curve showing the equivalence point and buffer …

Titrations · Intermediate (High School) · 50 min · 6 steps

Vitamin C Titration with Iodine

Measuring ascorbic acid content in fruit juice

Determine the vitamin C (ascorbic acid) content of fresh fruit juice using an iodine titration with starch indicator.

Titrations · Intermediate (High School) · 40 min · 7 steps