Spectroscopy & Instrumentation
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Analytical instruments and spectroscopic techniques — UV-Vis, IR, NMR, mass spectrometry, and chromatography.
Introduction to Spectroscopy
Electromagnetic spectrum, photon energy, absorption vs emission, and Beer-Lambert law
UV-Visible Spectroscopy
UV-Vis principles, chromophores, conjugation, and quantitative analysis
Infrared Spectroscopy Techniques
IR absorption, functional group identification, fingerprint region, and FTIR
Nuclear Magnetic Resonance (NMR)
Chemical shift, spin-spin coupling, 1H and 13C NMR interpretation
Mass Spectrometry Techniques
EI, ESI, MALDI ionization, mass analyzers, and fragmentation patterns
X-Ray Diffraction and Crystallography
Bragg diffraction, crystal structure determination, and protein crystallography
Chromatography Principles
HPLC, GC, TLC — partition, adsorption, and ion-exchange chromatography
Electroanalytical Techniques
Potentiometry, voltammetry, coulometry, and ion-selective electrodes
Atomic Spectroscopy
AAS, AES, ICP-OES, ICP-MS — elemental analysis and detection limits
Modern Analytical Techniques
Raman spectroscopy, fluorescence, XPS, SEM-EDS, and hyphenated techniques