Chemistry Experiments

95 experiment protocols with step-by-step instructions

Difficulty	≤15 min	≤30 min	≤60 min	60+ min
Beginner (Middle School)	1	1	1	1
Intermediate (High School)	0	1	1	1
Advanced (University)	0	1	1	1
Professional (Lab)	0	0	0	1

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Beer-Lambert Law Verification

Demonstrating the linear relationship between absorbance and concentration

Verify the Beer-Lambert law by measuring the absorbance of CuSO₄ solutions at various concentrations and constructing a calibration curve.

Measuring blood glucose analog using the DNS method

Determine glucose concentration using the dinitrosalicylic acid (DNS) colorimetric method and understand reducing sugar chemistry.

Measuring phosphate levels in water using the molybdenum blue method

Determine the phosphate concentration in a water sample using the ammonium molybdate colorimetric method.

Quantifying protein concentration using Coomassie Brilliant Blue dye binding

Determine the protein concentration of an unknown sample using the Bradford assay and a BSA calibration curve.

Measuring dissolved iron concentration using thiocyanate colorimetry

Determine the concentration of iron(III) in a water sample using the thiocyanate colorimetric method.

Identifying artificial food colors by their absorption spectra

Determine the identity and concentration of artificial food dyes in commercial beverages by measuring their absorption spectra.

Resolving a two-component mixture using simultaneous equations

Determine the individual concentrations of two dyes in a mixed solution using absorbance measurements at two wavelengths and simultaneous equations.

Measuring aspirin content in commercial tablets by UV absorption

Determine the amount of acetylsalicylic acid (aspirin) in commercial tablets using UV spectrophotometry after hydrolysis to salicylate.