水中の鉄の定量

チオシアン酸比色法を使った溶解鉄濃度の測定

Colorimetry Intermediate (High School) 75 分 ~$10.00

目的

チオシアン酸比色法を用いて水試料中の鉄(III)濃度を決定する。

背景

Iron(III) ions react with thiocyanate ions (SCN⁻) to form the intensely red-colored complex [Fe(SCN)]²⁺. The intensity of the red color is proportional to the Fe³⁺ concentration, following the Beer-Lambert law. This method is widely used in water quality testing and environmental monitoring. Students learn both the analytical technique and the chemistry of complex ion formation.

安全上の警告

  • FeCl₃ solution stains skin and clothing
  • KSCN is harmful if swallowed
  • HNO₃ is corrosive — handle with care
  • Dispose of thiocyanate waste in designated containers

必要なPPE

goggles gloves lab_coat

材料

  • Iron(III) chloride (FeCl₃) standard (100 mL)
    0.01 M stock solution
  • Potassium thiocyanate (KSCN) (100 mL)
    0.1 M solution
  • Dilute HNO₃ (0.1 M) (50 mL)
    To maintain acidity
  • Water sample (50 mL)
    Unknown iron content
  • Distilled water (500 mL)
    For dilutions

器具

Colorimeter or spectrophotometer Cuvettes (matched pair) Volumetric flasks (50 mL, 7 total) Pipettes (1 mL, 5 mL, 10 mL) Pipette filler

手順

1

Prepare a series of standard Fe³⁺ solutions (0, 1, 2, 4, 6, 8, 10 ppm) by diluting the stock FeCl₃ solution in 50 mL volumetric flasks.

15 分
2

Add 5 mL of 0.1 M KSCN and 2 mL of dilute HNO₃ to each standard flask and the water sample. Make up to volume with distilled water.

10 分
3

Allow 5 minutes for full color development. The solutions should range from pale to deep red.

5 分
4

Set the colorimeter to 480 nm. Zero with the blank solution (0 ppm Fe³⁺ + KSCN).

5 分
5

Measure the absorbance of each standard solution and the water sample.

15 分
6

Plot the calibration curve (absorbance vs. concentration). Use it to determine the iron concentration in the water sample.

15 分
7

Calculate the iron concentration and evaluate whether the water meets drinking water standards (<0.3 ppm).

10 分

予想される結果

The calibration curve should be linear from 0 to 10 ppm. The deep red color of [Fe(SCN)]²⁺ intensifies with increasing Fe³⁺ concentration. The water sample's iron content is determined from the calibration curve.

後片付け

Collect all thiocyanate-containing solutions in labeled waste. Rinse all glassware thoroughly. Wipe down the colorimeter.

詳細

カテゴリ
Colorimetry
難易度
Intermediate (High School)
所要時間
75 分
推定費用
$10.00
ステップ数
7
材料
5

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Frequently Asked Questions

What is the objective of 水中の鉄の定量?
チオシアン酸比色法を用いて水試料中の鉄(III)濃度を決定する。
How difficult is 水中の鉄の定量?
This experiment is rated as Intermediate (High School). It takes approximately 75 minutes to complete.
What safety precautions are needed for 水中の鉄の定量?
Key safety precautions include: FeCl₃ solution stains skin and clothing; KSCN is harmful if swallowed; HNO₃ is corrosive — handle with care.
What materials are needed for 水中の鉄の定量?
The main materials required are: Iron(III) chloride (FeCl₃) standard, Potassium thiocyanate (KSCN), Dilute HNO₃ (0.1 M), Water sample, Distilled water.
What results should I expect from 水中の鉄の定量?
The calibration curve should be linear from 0 to 10 ppm. The deep red color of [Fe(SCN)]²⁺ intensifies with increasing Fe³⁺ concentration. The water sample's iron content is determined from the calibration curve.