分光光度法による食品着色料分析

吸収スペクトルで人工食品着色料を同定する

Colorimetry Intermediate (High School) 60 分 ~$8.00

目的

吸収スペクトルを測定することで市販飲料中の人工食品着色料の種類と濃度を決定する。

背景

Artificial food dyes have characteristic absorption spectra that serve as fingerprints for identification. FD&C Red No. 40 absorbs maximally at 504 nm, Blue No. 1 at 630 nm, and Yellow No. 5 at 428 nm. By scanning the absorption spectrum of a commercial beverage and comparing peak wavelengths with known dye spectra, students can identify which dyes are present and estimate their concentrations.

安全上の警告

  • Food dyes can stain clothing
  • Do not drink any samples after use in the lab
  • Handle cuvettes by the frosted sides only

必要なPPE

goggles lab_coat

材料

  • Standard food dye solutions (50 mL each)
    Red 40, Blue 1, Yellow 5 (0.01%)
  • Commercial beverages (50 mL each)
    2–3 colored drinks
  • Distilled water (200 mL)
    For dilutions and blank

器具

UV-Vis spectrophotometer (or colorimeter with multiple filters) Cuvettes (quartz or plastic) Pipettes Volumetric flasks (25 mL, 4 total)

手順

1

If using a spectrophotometer, scan each standard dye solution from 400 to 700 nm to identify the λmax (wavelength of maximum absorbance).

10 分
2

Record the λmax for each standard: Red 40 (≈504 nm), Blue 1 (≈630 nm), Yellow 5 (≈428 nm).

5 分
3

Dilute commercial beverage samples if too concentrated (absorbance should be below 1.0). Record all dilution factors.

10 分
4

Scan each beverage sample from 400 to 700 nm. Record the absorption spectrum.

15 分
5

Compare the beverage absorption peaks with the standard dye λmax values to identify which dyes are present.

10 分
6

If a calibration curve was prepared for a specific dye, calculate the dye concentration in each beverage (accounting for dilution).

10 分

予想される結果

Each beverage should show absorption peaks matching one or more standard food dyes. Green drinks typically contain both Blue 1 and Yellow 5. Orange drinks often contain Red 40 and Yellow 5.

後片付け

Pour dilute dye solutions down the drain with running water. Rinse cuvettes immediately to prevent staining.

詳細

カテゴリ
Colorimetry
難易度
Intermediate (High School)
所要時間
60 分
推定費用
$8.00
ステップ数
6
材料
3

その他の実験

グルコースの比色定量 60 min ランベルト-ベールの法則の検証 60 min リン酸塩の比色定量 90 min 分光光度法によるアスピリンの定量 75 min 比色タンパク質定量（Bradford法） 60 min 水中の鉄の定量 75 min 混合物の分光光度法分析 75 min

Frequently Asked Questions

What is the objective of 分光光度法による食品着色料分析?
吸収スペクトルを測定することで市販飲料中の人工食品着色料の種類と濃度を決定する。
How difficult is 分光光度法による食品着色料分析?
This experiment is rated as Intermediate (High School). It takes approximately 60 minutes to complete.
What safety precautions are needed for 分光光度法による食品着色料分析?
Key safety precautions include: Food dyes can stain clothing; Do not drink any samples after use in the lab; Handle cuvettes by the frosted sides only.
What materials are needed for 分光光度法による食品着色料分析?
The main materials required are: Standard food dye solutions, Commercial beverages, Distilled water.
What results should I expect from 分光光度法による食品着色料分析?
Each beverage should show absorption peaks matching one or more standard food dyes. Green drinks typically contain both Blue 1 and Yellow 5. Orange drinks often contain Red 40 and Yellow 5.