Спектрофотометрическое определение аспирина

Измерение содержания аспирина в коммерческих таблетках по поглощению в УФ-диапазоне

Colorimetry Advanced (University) 75 мин ~$10,00

Цель

Определить количество ацетилсалициловой кислоты (аспирина) в коммерческих таблетках методом УФ-спектрофотометрии после гидролиза до салицилата.

Предпосылки

Aspirin (acetylsalicylic acid) can be quantified by first hydrolyzing it to salicylic acid, which forms an intensely purple complex with iron(III) chloride. This complex absorbs strongly at 530 nm. By comparing the absorbance of hydrolyzed tablet solutions with a calibration curve of standard salicylic acid solutions, students determine whether commercial tablets contain the labeled amount of aspirin.

Предупреждения по безопасности

  • NaOH and HCl are corrosive
  • Do not ingest any laboratory samples
  • FeCl₃ stains skin and clothing
  • Handle hot solutions with care

Требуемые СИЗ

goggles gloves lab_coat

Материалы

  • Aspirin tablets (commercial) (3 tablets)
    325 mg or 500 mg labeled
  • Salicylic acid standard (1 g)
    For calibration
  • Iron(III) chloride (FeCl₃) solution (1%) (100 mL)
    Color reagent
  • NaOH (1M) (50 mL)
    For hydrolysis
  • HCl (1M) (50 mL)
    For acidification
  • Distilled water (500 mL)

Оборудование

Spectrophotometer Cuvettes Volumetric flasks (100 mL, 8 total) Hot plate or water bath Mortar and pestle Analytical balance Filter paper and funnel

Процедура

1

Prepare salicylic acid standards: 0, 5, 10, 20, 30, 40 ppm in 100 mL volumetric flasks.

10 мин
2

Crush one aspirin tablet and dissolve in 10 mL of 1M NaOH. Heat gently on a water bath for 15 minutes to hydrolyze the ester bond.

20 мин Handle hot solution carefully
3

Cool the solution, acidify with 10 mL of 1M HCl, and filter into a 100 mL volumetric flask. Dilute to an expected concentration within the calibration range.

10 мин
4

Add 5 mL of 1% FeCl₃ solution to each standard and sample flask. A purple color develops.

5 мин
5

Allow 5 minutes for full color development. Measure absorbance at 530 nm, blanking with the 0 ppm standard.

15 мин
6

Plot the calibration curve. Determine the salicylic acid concentration in the tablet solution, then calculate back to the original aspirin mass (accounting for hydrolysis stoichiometry and dilution).

15 мин

Ожидаемые результаты

The calibration curve should be linear. The calculated aspirin content should be close to the labeled amount (typically 95–105% of the stated dose). The purple FeCl₃ complex confirms salicylate presence.

Уборка

Neutralize acid/base waste before disposal. Collect FeCl₃ waste separately. Rinse all glassware thoroughly.

Детали

Категория
Colorimetry
Сложность
Advanced (University)
Продолжительность
75 мин
Прим. стоимость
$10,00
Шаги
6
Материалы
6

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Frequently Asked Questions

What is the objective of Спектрофотометрическое определение аспирина?
Определить количество ацетилсалициловой кислоты (аспирина) в коммерческих таблетках методом УФ-спектрофотометрии после гидролиза до салицилата.
How difficult is Спектрофотометрическое определение аспирина?
This experiment is rated as Advanced (University). It takes approximately 75 minutes to complete.
What safety precautions are needed for Спектрофотометрическое определение аспирина?
Key safety precautions include: NaOH and HCl are corrosive; Do not ingest any laboratory samples; FeCl₃ stains skin and clothing.
What materials are needed for Спектрофотометрическое определение аспирина?
The main materials required are: Aspirin tablets (commercial), Salicylic acid standard, Iron(III) chloride (FeCl₃) solution (1%), NaOH (1M), HCl (1M).
What results should I expect from Спектрофотометрическое определение аспирина?
The calibration curve should be linear. The calculated aspirin content should be close to the labeled amount (typically 95–105% of the stated dose). The purple FeCl₃ complex confirms salicylate presence.