Phosphate Buffer System

H2PO4− ⇌ HPO42− + H+

概述

The dihydrogen phosphate/hydrogen phosphate buffer system operates near pH 7.2, making it ideal for biological applications. With pKa2 = 7.2, it buffers effectively between pH 6.2 and 8.2. This is the buffer system used in phosphate-buffered saline (PBS), the most common buffer in biology.

参与者

角色 物质 系数 状态
反应物 Phosphoric Acid H₃PO₄ 1 (aq)

日常示例

Phosphate buffers help maintain the pH of many biological fluids and are used in nearly every molecular biology laboratory.

工业重要性

磷酸盐缓冲生理盐水（PBS）是细胞培养、蛋白质纯化和药物制剂中最广泛使用的缓冲液。

属性

类型
Acid-Base
可逆
是
能量
吸热

相关反应

Hydrochloric Acid and Barium Hydroxide Acetic Acid and Sodium Acetate Buffer System Acetic Acid Dissociation in Water Ammonia and Hydrochloric Acid Ascorbic Acid and Sodium Hydroxide Barium Hydroxide and Ammonium Thiocyanate (Endothermic) Carbonic Acid and Sodium Hydroxide Aluminum Hydroxide as Amphoteric Acid with NaOH Aluminum Chloride and Chloride Ion (Lewis Acid-Base) Formic Acid and Sodium Hydroxide

Frequently Asked Questions

What is the equation for Phosphate Buffer System?
The balanced equation is: H₂PO₄⁻ ⇌ HPO₄²⁻ + H⁺.
What type of reaction is Phosphate Buffer System?
Phosphate Buffer System is a acid-base reaction. It is reversible under certain conditions.
Is Phosphate Buffer System exothermic or endothermic?
Phosphate Buffer System is endothermic (absorbs energy).