Thử Nghiệm Tinh Bột-Iot Cho Carbohydrat
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Phát hiện tinh bột trong mẫu thực phẩm bằng dung dịch iot
Mục tiêu
Dùng thử nghiệm iot để nhận biết tinh bột trong các mẫu thực phẩm khác nhau và hiểu cơ sở phân tử của phức màu xanh đen.
Bối cảnh
The starch-iodine test is one of the most visually striking qualitative tests in chemistry. Iodine (I₂) forms a deep blue-black complex with amylose, the helical component of starch. The iodine molecules slip inside the amylose helix, creating a charge-transfer complex that absorbs light strongly. This test does not work with simple sugars (glucose, sucrose) or with amylopectin alone, making it specific for amylose-containing starch.
Cảnh báo an toàn
- Iodine solution stains skin and clothing
- Do not taste any samples after adding iodine
- Handle knife carefully when cutting food samples
PPE cần thiết
Vật liệu
-
Iodine solution (Lugol's) (10 mL)I₂/KI solution
-
Starch solution (1%) (10 mL)Positive control
-
Glucose solution (1%) (10 mL)Negative control
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Food samples (Various)Potato, bread, rice, apple, etc.
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Distilled water (50 mL)
Thiết bị
Quy trình
Place small amounts of each food sample and control solutions in separate wells of the spot plate.
Add 2–3 drops of Lugol's iodine solution to the starch solution (positive control). Observe the blue-black color.
Add 2–3 drops to the glucose solution (negative control). Observe that the color remains amber/brown.
Add 2–3 drops of iodine to each food sample. Record the color change observed.
Classify each sample as starch-positive (blue-black) or starch-negative (amber/brown). Record in a data table.
Kết quả dự kiến
Potato, bread, and rice should test strongly positive (deep blue-black). Apple and pure sugar solutions should remain amber/brown. The intensity of the blue color correlates with starch content.
Dọn dẹp
Dispose of iodine-treated samples in general waste. Rinse spot plates and pipettes with water.
Chi tiết
- Danh mục
- Qualitative Analysis
- Độ khó
- Beginner (Middle School)
- Thời gian
- 30 phút
- Chi phí ước tính
- $5,00
- Số bước
- 5
- Vật liệu
- 5