Chuẩn Độ Vitamin C với Iot

Đo hàm lượng axit ascorbic trong nước ép trái cây

Titrations Intermediate (High School) 40 phút ~$10,00

Mục tiêu

Xác định hàm lượng vitamin C (axit ascorbic) trong nước ép trái cây tươi bằng chuẩn độ iot với chỉ thị tinh bột.

Bối cảnh

Ascorbic acid reduces iodine to iodide ions in a 1:1 molar ratio. Starch indicator turns dark blue-black in the presence of excess iodine. When all the vitamin C is consumed, the next drop of iodine produces the blue-black color, marking the endpoint.

Cảnh báo an toàn

  • Iodine stains skin and clothing
  • Wear safety goggles
  • Handle iodine solution with care

PPE cần thiết

goggles lab_coat

Vật liệu

  • Fresh orange juice (200 mL)
    Freshly squeezed preferred
  • Iodine solution (100 mL)
    0.005M
  • Starch indicator solution (10 mL)
    1% solution
  • Distilled water (200 mL)

Thiết bị

50 mL burette Burette stand and clamp 250 mL Erlenmeyer flask 25 mL pipette Pipette filler White tile

Quy trình

1

Rinse and fill the burette with the standardized iodine solution. Record the initial volume.

3 phút Iodine stains
2

Pipette 25.0 mL of orange juice into the Erlenmeyer flask.

2 phút
3

Add 5 drops of starch indicator to the flask.

1 phút
4

Titrate by adding iodine solution slowly while swirling. The brown iodine color will disappear as it reacts with vitamin C.

8 phút
5

Near the endpoint, add drop by drop until a persistent dark blue-black color appears for 20 seconds.

3 phút
6

Record the final volume and repeat for concordant results.

15 phút
7

Calculate the mass of vitamin C per volume of juice. Compare with the labeled value.

5 phút

Kết quả dự kiến

Fresh orange juice typically contains 40-80 mg of vitamin C per 100 mL. The starch-iodine endpoint should be a sharp transition to blue-black. Older or heat-treated juice will show lower vitamin C content.

Dọn dẹp

Dispose of iodine waste in designated containers. Rinse all glassware with water. Clean any iodine stains with sodium thiosulfate solution.

Frequently Asked Questions

What is the objective of Chuẩn Độ Vitamin C với Iot?
Xác định hàm lượng vitamin C (axit ascorbic) trong nước ép trái cây tươi bằng chuẩn độ iot với chỉ thị tinh bột.
How difficult is Chuẩn Độ Vitamin C với Iot?
This experiment is rated as Intermediate (High School). It takes approximately 40 minutes to complete.
What safety precautions are needed for Chuẩn Độ Vitamin C với Iot?
Key safety precautions include: Iodine stains skin and clothing; Wear safety goggles; Handle iodine solution with care.
What materials are needed for Chuẩn Độ Vitamin C với Iot?
The main materials required are: Fresh orange juice, Iodine solution, Starch indicator solution, Distilled water.
What results should I expect from Chuẩn Độ Vitamin C với Iot?
Fresh orange juice typically contains 40-80 mg of vitamin C per 100 mL. The starch-iodine endpoint should be a sharp transition to blue-black. Older or heat-treated juice will show lower vitamin C content.