분광광도법으로 식품 색소 분석

흡수 스펙트럼으로 인공 식품 색소 확인하기

Colorimetry Intermediate (High School) 60 분 ~$8.00

목표

흡수 스펙트럼을 측정하여 시판 음료의 인공 식품 색소의 종류와 농도를 결정한다.

배경

Artificial food dyes have characteristic absorption spectra that serve as fingerprints for identification. FD&C Red No. 40 absorbs maximally at 504 nm, Blue No. 1 at 630 nm, and Yellow No. 5 at 428 nm. By scanning the absorption spectrum of a commercial beverage and comparing peak wavelengths with known dye spectra, students can identify which dyes are present and estimate their concentrations.

안전 경고

  • Food dyes can stain clothing
  • Do not drink any samples after use in the lab
  • Handle cuvettes by the frosted sides only

필수 개인 보호 장비

goggles lab_coat

재료

  • Standard food dye solutions (50 mL each)
    Red 40, Blue 1, Yellow 5 (0.01%)
  • Commercial beverages (50 mL each)
    2–3 colored drinks
  • Distilled water (200 mL)
    For dilutions and blank

장비

UV-Vis spectrophotometer (or colorimeter with multiple filters) Cuvettes (quartz or plastic) Pipettes Volumetric flasks (25 mL, 4 total)

실험 절차

1

If using a spectrophotometer, scan each standard dye solution from 400 to 700 nm to identify the λmax (wavelength of maximum absorbance).

10 분
2

Record the λmax for each standard: Red 40 (≈504 nm), Blue 1 (≈630 nm), Yellow 5 (≈428 nm).

5 분
3

Dilute commercial beverage samples if too concentrated (absorbance should be below 1.0). Record all dilution factors.

10 분
4

Scan each beverage sample from 400 to 700 nm. Record the absorption spectrum.

15 분
5

Compare the beverage absorption peaks with the standard dye λmax values to identify which dyes are present.

10 분
6

If a calibration curve was prepared for a specific dye, calculate the dye concentration in each beverage (accounting for dilution).

10 분

예상 결과

Each beverage should show absorption peaks matching one or more standard food dyes. Green drinks typically contain both Blue 1 and Yellow 5. Orange drinks often contain Red 40 and Yellow 5.

정리

Pour dilute dye solutions down the drain with running water. Rinse cuvettes immediately to prevent staining.

세부 정보

카테고리
Colorimetry
난이도
Intermediate (High School)
소요 시간
60 분
예상 비용
$8.00
단계
6
재료
3

더 많은 실험

분광광도법으로 아스피린 정량 75 min 혼합물의 분광광도법 분석 75 min 인산염의 비색 정량 90 min 포도당의 비색 정량 60 min 람베르트-베어 법칙 검증 60 min 비색 단백질 정량(브래드퍼드법) 60 min 물 속 철 함량 결정 75 min

Frequently Asked Questions

What is the objective of 분광광도법으로 식품 색소 분석?
흡수 스펙트럼을 측정하여 시판 음료의 인공 식품 색소의 종류와 농도를 결정한다.
How difficult is 분광광도법으로 식품 색소 분석?
This experiment is rated as Intermediate (High School). It takes approximately 60 minutes to complete.
What safety precautions are needed for 분광광도법으로 식품 색소 분석?
Key safety precautions include: Food dyes can stain clothing; Do not drink any samples after use in the lab; Handle cuvettes by the frosted sides only.
What materials are needed for 분광광도법으로 식품 색소 분석?
The main materials required are: Standard food dye solutions, Commercial beverages, Distilled water.
What results should I expect from 분광광도법으로 식품 색소 분석?
Each beverage should show absorption peaks matching one or more standard food dyes. Green drinks typically contain both Blue 1 and Yellow 5. Orange drinks often contain Red 40 and Yellow 5.